Journal: Cell

Article Title: A blood atlas of COVID-19 defines hallmarks of disease severity and specificity

doi: 10.1016/j.cell.2022.01.012

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Article Snippet: CD22 (HIB22)-159Tb , Fluidigm , Cat# 3159005B, RRID: AB_2892688.

Techniques: Mass Cytometry, Flow Cytometry, Recombinant, Staining, Selection, Antibody Labeling, Labeling, Isolation, Sample Prep, Luminex, Quantitative Proteomics, Generated, Gene Expression, Clone Assay, Marker, Expressing, Mass Spectrometry, Derivative Assay, RNA Sequencing, Sequencing, Illumina Sequencing, Software, Variant Assay

Journal: Cell

Article Title: Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches

doi: 10.1016/j.cell.2021.12.018

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Article Snippet: Anti-Human CD22 (REA340) APC , Miltenyi Biotec , 130-120-762; RRID: AB_2752186.

Techniques: Purification, Recombinant, Staining, cDNA Synthesis, Gene Expression, Software, Microscopy

Journal: Nature Communications

Article Title: Split-design approach enhances the therapeutic efficacy of ligand-based CAR-T cells against multiple B-cell malignancies

doi: 10.1038/s41467-024-54150-z

Figure Lengend Snippet: a Timeline of in vivo experiments. Consistent results were obtained in two independent experiments ( n = 5 mice). b Representative bioluminescence images of mice subjected to different treatments. Colors represent the luminescence intensity (red, highest; blue, lowest). c , d Quantification of the average radiance (p/s/cm /sr) of the luminescence, related to APRIL- ( c ) and BAFF-( d )-based CAR-T-cell therapy. Two-way ANOVA multiple comparisons in Dunnett correction were used to assess significance. e Evaluation of serum inflammatory cytokine release by ELISA 24 h after CAR-T-cell infusion. One-way ANOVA multiple comparisons in Tukey correction were used to assess significance. f , g Survival curves of the mice subjected to the indicated treatments. Survival curves were compared using the log-rank (Mantel‒Cox) test. h Timeline of in vivo experiments. Consistent results were obtained in two independent experiments ( n = 5 mice). i Representative bioluminescence images of mice subjected to different treatments. Colors represent the luminescence intensity (red, highest; blue, lowest). j Quantification of the average radiance (p/s/cm /sr) of the luminescence. Two-way ANOVA multiple comparisons in Dunnett correction were used to assess significance, comparing 9E10-IgG4m CAR-T (with Myc-BAFF) and CD19/CD22 CAR-T. k Evaluation of serum inflammatory cytokine release by ELISA 24 h after CAR-T-cell infusion. One-way ANOVA multiple comparisons in Dunnett correction were used to assess significance. l Assessment of the presence of persistent human CD3 + (hCD3 + ) T cells in peripheral blood by flow cytometry over a 3-week follow-up period. Two-way ANOVA multiple comparisons in Dunnett correction were used to assess significance, comparing 9E10-IgG4m CAR-T (with Myc-BAFF) with BAFF-CAR-T at each time point. m Survival curves of mice subjected to the indicated treatments, compared using the log-rank (Mantel‒Cox) test. All n represents biological replicates from different mice. Data in this figure are representative of one of two independent experiments. Error bars represent mean ± SEM. NS indicates not significant. Source data are provided in the Source Data file.

Article Snippet: For western blot, anti-human CD19 antibody (Boster, BM4935) and anti-human CD22 antibody (Boster, BM4178) were used to verify the KO efficiency .

Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Flow Cytometry

Journal: PLoS ONE

Article Title: A Novel Class of Anti-HIV Agents with Multiple Copies of Enfuvirtide Enhances Inhibition of Viral Replication and Cellular Transmission In Vitro

doi: 10.1371/journal.pone.0041235

Figure Lengend Snippet: Comparing the potency of P4/D10, cP4/D10, h734-(T20) 4 , and hLL2-(T20) 4 for neutralization of HIV-1 IIIB in Jurkat T cells dosed at 50 TCID 50 (A) and 100 TCID 50 (B), and HIV-1 6794 in PBMCs dosed at 50 TCID 50 (C) and 100 TCID 50 (D).

Article Snippet: For measuring all hLL2-containing species, plates were coated with AffiniPure Goat Anti-Human IgG, F(ab′) 2 Fragment Specific (Jackson ImmunoReserach, West Grove, PA) and the captured antibodies were probed with a rat anti-idiotype mAb to hLL2 (WN; Immunomedics, Inc.), followed by Peroxidase AffiniPure F(ab′) 2 Frag Goat anti-Rat IgG, FcR Frag Spec (Jackson ImmunoReserach).

Techniques: Neutralization

Journal: PLoS ONE

Article Title: A Novel Class of Anti-HIV Agents with Multiple Copies of Enfuvirtide Enhances Inhibition of Viral Replication and Cellular Transmission In Vitro

doi: 10.1371/journal.pone.0041235

Figure Lengend Snippet: Estimated EC 50 (nM) a .

Article Snippet: For measuring all hLL2-containing species, plates were coated with AffiniPure Goat Anti-Human IgG, F(ab′) 2 Fragment Specific (Jackson ImmunoReserach, West Grove, PA) and the captured antibodies were probed with a rat anti-idiotype mAb to hLL2 (WN; Immunomedics, Inc.), followed by Peroxidase AffiniPure F(ab′) 2 Frag Goat anti-Rat IgG, FcR Frag Spec (Jackson ImmunoReserach).

Techniques:

Journal: PLoS ONE

Article Title: A Novel Class of Anti-HIV Agents with Multiple Copies of Enfuvirtide Enhances Inhibition of Viral Replication and Cellular Transmission In Vitro

doi: 10.1371/journal.pone.0041235

Figure Lengend Snippet: Concentrations of intact hLL2-(T20) 4 ( • ) and all hLL2-containing species (•) in serum samples collected from mice at 30-min, 6-h, 24-h, and 72-h, post-injection of hLL2-T20, compared with concentrations of hLL2 (•) in serum samples collected from mice at the same time points post-injection of hLL2.

Article Snippet: For measuring all hLL2-containing species, plates were coated with AffiniPure Goat Anti-Human IgG, F(ab′) 2 Fragment Specific (Jackson ImmunoReserach, West Grove, PA) and the captured antibodies were probed with a rat anti-idiotype mAb to hLL2 (WN; Immunomedics, Inc.), followed by Peroxidase AffiniPure F(ab′) 2 Frag Goat anti-Rat IgG, FcR Frag Spec (Jackson ImmunoReserach).

Techniques: Injection